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                    當(dāng)前位置:主頁(yè) > 產(chǎn)品中心 > > 抗體/抗原 >Anti-Ubiquitin (linkage-specific K63) EPR8590-448 antibody 抗泛素抗體

                    Anti-Ubiquitin (linkage-specific K63) EPR8590-448 antibody 抗泛素抗體

                    簡(jiǎn)要描述:Anti-Ubiquitin (linkage-specific K63) [EPR8590-448] antibody 抗泛素抗體
                    貨號(hào):ab179434
                    廠(chǎng)家:Abcam公司
                    規(guī)格:40ul/100ul
                    保存溫度:-20℃ 和 2~8℃
                    貨期:現(xiàn)貨2~5天,期貨2-3周

                    • 產(chǎn)品型號(hào):ab179434
                    • 廠(chǎng)商性質(zhì):代理商
                    • 更新時(shí)間:2017-01-26
                    • 訪(fǎng)  問(wèn)  量:3422
                    詳細(xì)介紹

                    咨詢(xún):  (重慶) (貴陽(yáng))

                    Anti-Ubiquitin (linkage-specific K63) [EPR8590-448] antibody 抗泛素抗體產(chǎn)品信息:

                     Product nameAnti-Ubiquitin (linkage-specific K63) [EPR8590-448] antibodySee all Ubiquitin primary antibodies

                        Description

                        Rabbit monoclonal [EPR8590-448] to Ubiquitin (linkage-specific K63)

                        Tested applicationsWB, IHC-P, ICC/IF more details

                        Species reactivity

                        Reacts with: Mouse, Rat, Human

                        Positive control

                            K63-linked-Ub2-7 recombinant protein; Human kidney carcinoma tissue.

                    Properties

                        FormLiquid

                        Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

                        PurityTissue culture supernatant

                        ClonalityMonoclonal

                        Clone numberEPR8590-448

                        IsotypeIgG

                        Research Areas

                            Neuroscience

                            Neurology process

                            Neurodegenerative disease

                            Alzheimer's disease

                            Tangles & Tau

                            Epigenetics and Nuclear Signaling

                            Chromatin Modifying Enzymes

                            Ubiquitylation

                            Epigenetics and Nuclear Signaling

                            DNA / RNA

                            DNA Damage & Repair

                            DNA Damage Response

                            Other

                            Cell Biology

                            Proteolysis / Ubiquitin

                            Proteasome / Ubiquitin

                            Ubiquitin

                            Neuroscience

                            Neurology process

                            Neurodegenerative disease

                            Other

                            Epigenetics and Nuclear Signaling

                            Ubiquitin & Ubiquitin Like Modifiers

                            Ubiquitin like Modifiers

                    Applications

                    Our Abpromise guarantee covers the use of ab179434 in the following tested applications.

                    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

                    Application      Notes

                    WB           WB: 1/1000 - 1/5000. Predicted molecular weight: 77 kDa.

                    IHC-P       IHC-P: 1/100 - 1/250.

                    ICC/IF      ICC/IF: 1/100 - 1/250.

                    Application notesIs unsuitable for ,Flow Cyt or IP.

                    Target

                        RelevanceFunction: Ubiquitin exists either covalently attached to another protein, or free (unanchored). When covalently bound, it is conjugated to target proteins via an isopeptide bond either as a monomer (monoubiquitin), a polymer linked via different Lys residues of the ubiquitin (polyubiquitin chains) or a linear polymer linked via the initiator Met of the ubiquitin (linear polyubiquitin chains). Polyubiquitin chains, when attached to a target protein, have different functions depending on the Lys residue of the ubiquitin that is linked: Lys-6-linked may be involved in DNA repair; Lys-11-linked is involved in ERAD (endoplasmic reticulum-associated degradation) and in cell-cycle regulation; Lys-29-linked is involved in lysosomal degradation; Lys-33-linked is involved in kinase modification; Lys-48-linked is involved in protein degradation via the proteasome; Lys-63-linked is involved in endocytosis, DNA-damage responses as well as in signaling processes leading to activation of the transcription factor NF-kappa-B. Linear polymer chains formed via attachment by the initiator Met lead to cell signaling. Ubiquitin is usually conjugated to Lys residues of target proteins, however, in rare cases, conjugation to Cys or Ser residues has been observed. When polyubiquitin is free (unanchored-polyubiquitin), it also has distinct roles, such as in activation of protein kinases, and in signaling. Similarity: Belongs to the ubiquitin family. Contains 3 ubiquitin-like domains.

                        Cellular localizationCell Membrane, Cytoplasmic and Nuclear

                        Database links

                            Entrez Gene: 7314 Human

                            Entrez Gene: 22187 Mouse

                            Entrez Gene: 192255 Rat

                            Omim: 191339 Human

                            SwissProt: P0CG47 Human

                            SwissProt: P0CG48 Human

                            SwissProt: P62988 Human

                            SwissProt: P0CG49 Mouse

                    Anti-Ubiquitin (linkage-specific K63) [EPR8590-448] antibody images

                        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polyubiquitin-C [EPR8590-448] antibody (ab179434)

                        Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Polyubiquitin-C [EPR8590-448] antibody (ab179434)

                        Immunohistochemical analysis of paraffin-embedded Human kidney carcinoma tissue labeling K63-linkage specific polyubiquitin with ab179434 at 1/100 dilution.

                        Western blot - Anti-Polyubiquitin-C [EPR8590-448] antibody (ab179434)

                        Western blot - Anti-Polyubiquitin-C [EPR8590-448] antibody (ab179434)

                        All lanes : Anti-Ubiquitin (linkage-specific K63) [EPR8590-448] antibody (ab179434) at 1/1000 dilution

                        Lane 1 : Ubiquitin recombinant protein

                        Lane 2 : K6-linked-Ub2 recombinant protein

                        Lane 3 : K11-linked-Ub2 recombinant protein

                        Lane 4 : K27-linked-Ub2 recombinant protein

                        Lane 5 : K29-linked-Ub2 recombinant protein

                        Lane 6 : K33-linked-Ub2 recombinant protein

                        Lane 7 : K48-linked-Ub2-7 recombinant protein

                        Lane 8 : K63-linked-Ub2-7 recombinant protein

                        Lysates/proteins at 0.02 µg per lane.

                        Predicted band size : 77 kDa

                    公司介紹:

                        Abcam位于英國(guó)的劍橋科學(xué)園,成立于1998年,專(zhuān)門(mén)生產(chǎn)和分銷(xiāo)研究型抗體。主要?jiǎng)?chuàng)立人Jonathan Milner和實(shí)驗(yàn)室同事構(gòu)思成立一間了解科研人員需要的網(wǎng)上抗體公司,理念是Abcam銷(xiāo)售質(zhì)量高的抗體,并提供zui全面、誠(chéng)實(shí)、時(shí)新的說(shuō)明書(shū),快速的送貨服務(wù),及高效率的顧客和服務(wù)。我們的在線(xiàn)目錄(www.abcam.cn) 已有差不多100,000種抗體和試劑,并不斷添加,供應(yīng)予百多個(gè)國(guó)家。Abcam200511月在倫敦證券交易所上市,在美國(guó)馬薩諸塞州、日本東京及香港均設(shè)有分公司。

                        Abcam 一直致力加強(qiáng)產(chǎn)品線(xiàn),為使研究員更容易找到蛋白質(zhì)研究試劑產(chǎn)品,在2011并購(gòu)了美國(guó)的MitoSciences公司,加強(qiáng)了免疫分析方面的產(chǎn)品供應(yīng);同年也并購(gòu)了英國(guó)的Ascent Scientific 公司,開(kāi)展了生化試劑的供應(yīng)。在2012年,Abcam并購(gòu)了美國(guó)的Epitomics 公司,成為一家有的RabMAbs® 供應(yīng)商。

                        Abcam 的目標(biāo)是給世界上的抗體建立zui大的在線(xiàn)目錄,為各地科學(xué)家提供*產(chǎn)品,成為各國(guó)科學(xué)界的重要伙伴。我們?yōu)樗挟a(chǎn)品提供來(lái)使客戶(hù)獲得預(yù)期的結(jié)果。為提供高質(zhì)量的抗體來(lái)指向盡可能多的靶蛋白,我們盡所能在盡可能多的應(yīng)用和物種中檢測(cè)每種抗體。我們相信誠(chéng)信才是上策,在我們的上會(huì)發(fā)布有關(guān)我們每一種產(chǎn)品的盡可能多的信息。

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                    更多詳情,請(qǐng):     

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