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Origen 二甲基亞砜DMSO及DMSO-DEX40供應
美國Origen
DMSO 屬于非質子極性溶劑，常用于化學反應、 PCR反應以及在細胞、組織和器官的保存中用作玻璃化低溫冷凍防護劑。 DMSO用于細胞冷凍培養(yǎng)基內，可以保護細胞免受冰晶引起的機械性損傷。它也可以用于主培養(yǎng)、亞培養(yǎng)、重組異倍體、雜交瘤等系列細胞株，胚胎干細胞（ ESC）和造血干細胞的冷凍保藏。另外常常與 BSA或FBS混合使用。
用 途：
（1） DMSO廣泛應用在人、動物細胞株及細菌噬菌體λ的低溫防護中，制備 DMSO溶液用來冷凍細胞的步驟如下：
A.配制冷凍培養(yǎng)基：含有細胞培養(yǎng)用培養(yǎng)基、 10-20% 血清和 5-10% DMSO；
B.用胰酶或其他合適的方法消化貼壁細胞；（細胞處于對數(shù)增長期）
C.低速離心（4℃，250×g, 10min）收集細胞，吸去培養(yǎng)基；
D.用冷凍培養(yǎng)基懸浮細胞，使細胞密度為 106 -107 cells/ml；
E.平均分配細胞，裝入凍存管內；
F.依照標準凍存程序冷凍細胞，凍存在 -70℃或更低的溫度內；
（2）細胞融合時，40-50% PEG 溶液內加入10% DMSO；
（3）有文獻報道 DMSO作為緩沖液的成分，用于以下的實驗：Poly（A+）RNA 的純化， E. coli感受態(tài)細胞轉化、 PCR反應、 cDNA文庫的擴增、 DNA測序、 DEAE-葡聚糖介導的細胞轉染、聚凝胺介導的 DNA轉染；
（4）有相應的程序描述到用 DMSO回收膜過濾后的 DNA，用于接下來的 PCR擴增反應；
（5）用于 DNA測序的毛細管電泳技術將 5% DMSO (v/w)混入 2 M尿素中，如果需要可以調整 DMSO濃度到100%。
（6）有研究報道不同濃度的 DMSO與寡核苷酸的熔點溫度關系；
（7）有研究描述利用 DMSO提高雜交瘤細胞中單克隆抗體的產量； 調查發(fā)現(xiàn)低溫冷凍前用含有 DMSO的培養(yǎng)基高溫培養(yǎng)雜交瘤細胞。
（8）文獻報道利用 DMSO修飾蛋白中的磷酸絲氨酸和磷酸蘇氨酸殘基用作磷酸化狀態(tài)的質譜分析（ MS）；
（9）文獻報道水和 DMSO中的亮脯利特（Leuprolide）降解作用；
互溶性：
? *互溶： LDPE, HDPE，聚丙烯 , PPCO, 聚甲基戊烯，尼龍， teflon FEP
? 中度互溶：聚苯乙烯， ECTFE/ETFE
? 不 互 溶：聚砜 ,， flexible and rigid PVC tubing，聚碳酸脂
注意事項：
僅用于實驗室，不可用于藥品、家居和其他用處 。
使用說明：
DMSO 易于水混合（ 1 ml DMSO + 1 ml H2O）形成清澈、無色的溶液。易吸潮，免暴露在潮濕環(huán)境。
儲存 /穩(wěn)定性：
DMSO 容易過冷并且室溫下緩慢融化，收到的產品應為固體并非液體狀態(tài)。固化的 DMSO放在室溫下可以再次液化，對其本身無損害。屬于熱穩(wěn)定化合物，在高達 100℃的酸、堿及中性溶液中保持穩(wěn)定。溫度接近其沸點（189℃），在堿性和中性溶液中仍保持穩(wěn)定。 150℃加熱 24h，其純度損失小于0.1%。
要制備濾膜除菌的 DMSO溶液，推薦使用 teflon或 nylon膜，盡量不用醋酸纖維素膜。
產品基本信息：
1、CryoPur DMSO
OriGen manufactures sterile filtered CryoPurTM 100% DMSO and CryoPur-DTM DMSO/Dextran.
The solutions are packaged in syringes or vials. Both packages are shatter resistant. All syringes are shipped in a tray to prevent any damage while in transit, and are fitted with a stopcock, shipped with the actuator blocked for safety.
Our DMSO products are CE marked and certified USP and Pharmacopeia Europa Standards.
VIALS
CryoPurTM: 100% DMSO
Stock Number Product Description Stock Item European Reference Number
CP-05 100% DMSO, 5ml Fill Volum V10005
CP-10 100% DMSO, 10ml Fill Volume X V10010
CP-50 100% DMSO, 50ml Fill Volume X V10050
CP-70 100% DMSO, 70ml Fill Volume X V10070
CP-100 100% DMSO, 100ml Fill Volume X V10100
CryoPur-DTM: 55% w/v DMSO, Buffered with 5% w/v Dextran-40
(*CryoPur-D is a mixture of pure DMSO, Dextran-40 and Water for Injection, with the resulting solution being 55% w/v DMSO, 5% Dextran-40, and the remainder water for injection)
Stock Number Product Description Stock Item European Reference Number
CD-05 DMSO/Dextran/remainder water, 5ml fill volume
CD-07 DMSO/Dextran/remainder water, 7.2ml fill volume X V504007
CD-35 DMSO/Dextran/remainder water, 35ml fill volume X V504035
CD-50 DMSO/Dextran/remainder water, 50ml fill volume X V504050
CD-70 DMSO/Dextran/remainder water, 70ml fill volume V504070
CD-100 DMSO/Dextran/remainder water, 100ml fill volume X V5040100
CryoPur-STM: 55% w/v DMSO, Buffered with 5% w/v Dextran-40
(*CryoPur-S is a mixture of pure DMSO, Dextran-40 and remainder 0.72% Saline, with the resulting solution being 55% w/v DMSO, 5% Dextran-40, and the remainder saline.)
Stock Number Product Description Stock Item European Reference Number
CDS-50B DMSO/Dextran/saline, 50ml fill volume V204850-S7
CDS-100B DMSO/Dextran/saline, 100ml fill volume V2048100-S7
SYRINGES
CryoPurTM: 100% DMSO
Stock Number Product Description Stock Item European Reference Number
SP-10 100% DMSO, 10ml in 20ml syringe, stopcock and SCD tubing X S10010
SP-20 100% DMSO, 20ml in 20ml syringe, stopcock and SCD tubing X
SP-30 100% DMSO, 30ml in 60ml syringe, stopcock and SCD tubing X S10030
SP-50 100% DMSO, 50ml in 60ml syringe, stopcock and SCD tubing X S10050
SP-10-1 100% DMSO, 10ml in 20ml syringe, stopcock and cap S10010SC
SP-50-1 100% DMSO, 50ml in 60ml syringe, stopcock and cap S10050SC
CryoPur-DTM: 55% w/v DMSO, Buffered with 5% w/v Dextran-40
(*CryoPur-D is a mixture of pure DMSO, Dextran-40 and Water for Injection, with the resulting solution being 55% w/v DMSO, 5% Dextran-40, and the remainder water for injection)
Stock Number Product Description Stock Item European Reference Number
SD-05 DMSO/Dextran/remainder water, 5ml in 20ml syringe, stopcock and SCD Tubing S504005
SD-06 DMSO/Dextran/remainder water, 6ml in 20ml syringe, stopcock and SCD Tubing S504006
SD-07 DMSO/Dextran/remainder water, 7.2ml in 20ml syringe, stopcock and SCD Tubing X S504007
SD-10 DMSO/Dextran/remainder water, 10ml in 20ml syringe, stopcock and SCD Tubing X S504010
SD-12 DMSO/Dextran/remainder water, 12ml in 20ml syringe, stopcock and SCD Tubing S504012
SD-10-1 DMSO/Dextran/remainder water, 10ml in 20ml syringe, stopcock and cap S504010SC
CryoPur-STM: 55% w/v DMSO, Buffered with 5% w/v Dextran-40
(*CryoPur-S is a mixture of pure DMSO, Dextran-40 and remainder 0.9% Saline, with the resulting solution being 55% w/v DMSO, 5% Dextran-40, and the remainder saline.)
Stock Number Product Description Stock Item European Reference Number
SDS-05A-B DMSO/Dextran/saline, 5ml fill volume + 2ml air in 20ml syringe, stopcock and SCD Tubing
SDS-15A DMSO/Dextran/saline, 15ml in 20ml syringe, stopcock and SCD tubing SDS-20A DMSO/Dextran/saline, 20ml in 20ml syringe, stopcock and SCD tubing
CryoPurTM: 80% DMSO, remainder water for injection
Stock Number Product Description Stock Item European Reference Number
SPW-10C DMSO, remainder water, 10ml in 20ml syringe, stopcock and SCD tubing SPW-30C DMSO, remainder water, 30ml in 60ml syringe, stopcock and SCD tubing SPW-50C DMSO, remainder water, 50ml in 60ml syringe, stopcock and SCD tubing
Storage
100% DMSO: +20 to 30°C (freezes/solidifies at 18°C, DMSO/Dextran: +2 to 8°C
Packaging
10ml vials: 12 to a case, larger vials: 6 to a case, syringes sold individually
Custom Sizes
Custom packages and formulations are possible, please enquire. Minimum order volumes may apply. 8-12 week lead time on custom items.
USA: For research use only.
其他：
Cryostore:Freezing Bags Freeze volumemin-max,10-30mL 凍存袋 24袋/箱 OriGen BioMedical CS50
Cryostore:Freezing Bags Freeze volumemin-max,30-70mL 凍存袋 24袋/箱 OriGen BioMedical CS250
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